Ok, this is my method (without referenced because I still need to finish citing)
Materials/Equipment needed: 30 Petri dishes or Petri dish substitute; 5 kitchen knives; (at least) 2 chopping boards; mm ruler; magnifying glass; camera; paper/ fabric face mask; organic materials including fresh samples of beef, chicken, an apple, an orange, a beetroot, a stick of celery, wholemeal and white bread, and a hard and soft cheese.
Method:
(Procedural Format):
1. Collect all materials and equipment.
2. Thoroughly wash kitchen knives and chopping boards in very hot, soapy water.
3. Cut two pieces, approx. 4cm x 4cm x 1cm, out of each organic material, using a different knife for each category of material (i.e. grain, fruit, etc) and using an entirely separate chopping board for meat.
4. Place each piece in a separate Petri dish, and ensure the face mask is being worn in order to prevent contamination. If possible, allow for some form of ventilation in each Petri dish.
5. Label each Petri dish appropriately. Separate samples into two groups of 10 samples, each group containing one of each type of organic material.
6. Place one group somewhere with access to sunlight, and label as Group 1. Place the other box in a place with little light, and label it as Group 2.
7. Measure the length/diameter, of fungal growth on each sample at a set time using a mm ruler and magnifying glass, take a photo of each sample and record any relevant observations. Do the same everyday for a period of 7 days/1 week (or less if clear and conclusive results can be seen before then). Record results in a table similar to Table 1.
8. Repeat steps 1-6, keeping the same time frame for the experiment as previously used.
For the Petri dishes, I am definitely using plastic plates instead.
Tuesday, May 11, 2010
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